| 摘要: |
| 病原菌美人鱼发光杆菌杀鱼亚种(Photobacterium damselae subsp. piscicida)分离自发病的卵形鲳鲹(Trachinotus ovatus), 本实验利用腹腔注射和浸泡的感染途径, 观察卵形鲳鲹发光杆菌病的病理变化。感染发病鱼呈现急性和慢性临床症状, 主要急性症状为鳃盖周围轻微出血, 腹腔积水和内脏器官多灶性坏死; 主要慢性症状为脾脏、肾脏和心脏内能观察到直径为0.5~1.0 mm 的白色粟米样结节; 利用光学显微镜和透射电镜观察的组织病理显示: 急性病理症状主要表现为鳃、肝和肾发生变性及凝固性坏死, 肾管微绒毛紊乱, 线粒体的嵴脱落, 脾淋巴细胞增生, 核染色质边集, 心肌细胞发生多灶性坏死,线粒体增生, 肠道的病变较轻微。慢性病理症状主要表现为鳃丝上皮细胞坏死, 脾淋巴细胞线粒体、高尔基体和内质网溶解, 肾小管上皮细胞的微绒毛脱落, 心肌纤维Z 带排列紊乱, 线粒体变性, 肝脏、肾脏、脾脏、心脏和肠道出现典型的肉芽肿病变。相比之下, 脾脏、肾脏和心脏的病变是所有器官中最严重的。 |
| 关键词: 卵形鲳鲹(Trachinotus ovatus) 美人鱼发光杆菌杀鱼亚种(Photobacterium damselae subsp. piscicida) 病理学 |
| DOI： |
| 分类号: |
| 基金项目:中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2008TS08; 2008YD02); 广东省科技计划项目(2010B020309002) |
|
| Histopathological analysis of golden pompanoTrachinotus ovatus infected with Photobacterium damselae subsp. piscicida |
|
|
| Abstract: |
| The pathogen Photobacterium damselae subsp. piscicida was isolated from the diseased Trachinotus ovatus. The aim of the study is to observe the pathological changes in T. ovatus after intraperitoneal injection or immersion routes of infection with Photobacterium damselae subsp. piscicida. The infected fishes exhibited acute and/or chronic symptoms, according to the lesional degree. The acute lesions includes slight hemorrhage around the gill covers, abdominal dropsy and multifocal necrosis in the internal organs. The chronic lesions are mainly small white miliary lesions with 0.5～1 mm in diameter observed in spleen, kidney and heart. The histopathological changes were observed under optical microscope and transmission electron microscope. In fishes with acute lesions, degeneration and coagulation necrosis in the gill, liver and kidney, microvillus disorder and mitochondrial cristae destruction in renal tubule, hyperplasia of macrophages and chromatin clumping of lymphocytes in the spleen, and hyperplasia of mitochondrion or multifocal necrosis in the heart were most pronounced in infected fish. Few or no lesions were observed in intestine. Fishes with chronic lesions suffered necrosis of the gill epithelium, disaggregation of mitochondria, Golgi body and endoplasmic reticulum in the spleen. The renal tubule epithelium microvillus underwent desquamation, meanwhile, the Z bands in myofibrils became disorder and mitochondria underwent denaturation in the heart. Typical chronic granulomatous lesions were also observed mainly in spleen, kidney, heart, liver and intestine. Generally, the most significant histopathological changes were detected in spleen, kidney and heart. |
| Key words: Trachinotus ovatus Photobacterium damselae subsp. piscicida histopathology |
