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引用本文:马蕊,阿尔祖古丽·买买提吐尔逊,张卫红.新疆一种琥珀螺线粒体基因组测序分析[J].海洋科学,2022,46(10):159-166.
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新疆一种琥珀螺线粒体基因组测序分析
马蕊, 阿尔祖古丽·买买提吐尔逊, 张卫红
新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室, 新疆 乌鲁木齐 830046
摘要:
本研究利用高通量测序技术获得采自新疆昭苏琥珀螺科一未知种的线粒体全基因组序列。该种琥珀螺线粒体基因组全长14219bp,由13个蛋白质编码基因,22个tRNA基因,2个rRNA基因及1个控制区组成。基因组中AT含量为76.43%,GC含量为23.57%,表现出明显的AT偏好性。13个PCGs多使用ATG为起始密码子,TAA为终止密码子。tRNA基因中tRNA-Ser1tRNA-Ser2缺少DHU臂,其中tRNA-Ser1在缺失的DHU臂处有一个小的茎环结构。非编码区位于COX3tRNA-Ile之间,长度为58bp。琥珀螺科中现有腐败琥珀螺Succineaputris五家渠尖缘螺OxylomawujiaquensisOmalonyxunguis的线粒体基因组被报道。三者的基因排列顺序和结构基本相似。昭苏琥珀螺未知种的基因排列顺序与它们有所不同。昭苏琥珀螺未知种的tRNA-TyrtRNA-Trp基因位于COX2tRNA-Gly之间,上述三种tRNA-TyrtRNA-Trp基因位于tRNA-Ser2tRNA-Ser1之间。系统发育树结果显示,琥珀螺科的四种聚为一支,为单系群,其中腐败琥珀螺和Omalonyxunguis亲缘关系更近。本研究结果支持昭苏琥珀螺未知种为中国一未报道的属种,但要确定其是否为一新属新种尚需更多的分子和形态数据。
关键词:  新疆  琥珀螺科  高通量测序  线粒体基因组
DOI:10.11759/hykx20211022001
分类号:Q959.212
基金项目:新疆维吾尔自治区自然科学基金项目(2020D01C043)
Complete mitochondrial genome of a succineid species from Xinjiang
MA Rui, AERZUGULI Maimaitituerxun, ZHANG Wei-hong
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
Abstract:
The complete mitochondrial genome of an unknown succineid species from Zhaosu, Xinjiang, was obtained using high-throughput sequencing technology. The mitochondrial genome of this species was 14, 219 bp in length with a high AT bias of 76.43%, containing 13 protein coding genes (PCGs), 22 tRNA genes, two rRNA genes and one control region. Most PCGs used ATG as the start codon and TAA as the stop codon. Among the tRNA genes, tRNA-Ser1 and tRNA-Ser2 lacked a DHU arm, and tRNA-Ser1 had a small stem-loop structure at the location of the missing DHU arm. The control region was 58 bp in length and was located between COX3 and tRNA-Ile. The complete mitogenome of Succinea putris, Oxyloma wujiaquensis, and Omalonyx unguis has been reported in the family Succineidae. The gene arrangement and structures of these three species were basically the same but different from the unknown species from Zhaosu. The tRNA-Tyr and tRNA-Trp genes of the species from Zhaosu were located between COX2 and tRNA-Gly, while the two genes of the three species were located between tRNA-Ser2 and tRNA-Ser1. The phylogenetic tree revealed that four species of Succineidae were clustered in one clade, suggesting a monophyletic group, whereas Succinea putris and Omalonyx unguis were more closely related than the other two species. The results of this study support that the succineid species from Zhaosu is an undescribed species in China. However, more molecular and morphological data are needed to determine whether this is a new genus and species.
Key words:  Xinjiang  Succineidae  high-throughput sequencing  mitochondrial genome
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