| 引用本文: | 吴琦汉,滕爽爽,李敏,张翔,肖国强,张建设,陈丽芝.泥蚶哈维氏弧菌抗性性状遗传参数的估计[J].海洋科学,2025,49(3):107-115. |
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| 泥蚶哈维氏弧菌抗性性状遗传参数的估计 |
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吴琦汉1,2, 滕爽爽2, 李敏2, 张翔2, 肖国强1,2, 张建设1, 陈丽芝3
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1.浙江海洋大学国家海洋设施养殖工程技术研究中心, 浙江 舟山 316022;2.浙江省海洋水产养殖研究所, 全省近岸生物种质资源保护与利用重点实验室, 浙江-泰国海洋技术联合实验室, 温州市海洋生物遗传育种重点实验室, 浙江 温州 325000;3.三门县水产技术推广站, 浙江 三门 317100
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| 摘要: |
| 为探究在哈维氏弧菌(Vibrio harveyi)感染下, 泥蚶(Tegillarca granosa)抗性性状的遗传参数, 作者通过哈维氏弧菌浸泡感染的方法, 对22个泥蚶家系进行人工感染实验, 采用3种遗传评定模型对泥蚶生长性状与抗性性状的遗传参数进行评估。实验结束时, 泥蚶的死亡率接近70%, 家系间的存活率存在显著差异(6.7%~60%)。结果显示, 抗性性状遗传力范围为(0.17±0.05)~(0.20±0.07), 属于中低遗传力。利用Pearson和Spearman相关对各家系育种值进行相关性分析, 3种模型估算的相关系数范围为0.953~0.998, 呈极显著的正相关, 说明3种模型在泥蚶抗性性状遗传参数的估算能力相似。生长性状与抗性性状之间表型相关和遗传相关分别为-0.069~-0.111和-0.412~-0.545, 均表现为不显著的负相关(P>0.05)。上述结果表明, 泥蚶抗性性状属于中低遗传力性状, 适合采用家系选育的方式对该性状进行选育。两性状间的遗传相关性没有显著性差异, 表明生长性状和抗性性状在育种中可分别选育。该项研究补充了泥蚶抗哈维氏弧菌遗传参数的相关性内容, 为选育泥蚶抗哈维氏弧菌优良品系提供了参考。 |
| 关键词: 泥蚶(Tegillarca granosa) 哈维氏弧菌(Vibrio harveyi) 遗传力 遗传相关 抗性性状 |
| DOI：10.11759/hykx20241210004 |
| 分类号:S968.3 |
| 基金项目:台州市重大农业科技研发攻关计划项目(NYJBGS202203); 现代农业产业技术体系建设专项(CARS-49); 浙江省自然科学基金资助项目(LY23C190006) |
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| Estimation of genetic parameters of the resistance traits of Tegillarca granosa to Vibrio harveyi |
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WU Qihan1,2, TENG Shuangshuang2, LI Min2, ZHANG Xiang2, XIAO Guoqiang1,2, ZHANG Jianshe1, CHEN Lizhi3
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1.National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China;2.Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Sino-Thai Joint Laboratory of Marine Technology, Wenzhou Key Laboratory of Marine Biological Genetics and Breeding, Wenzhou 325000, China;3.Extension Station for Aquaculture Technique, Sanmen 317100, China
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| Abstract: |
| To explore the genetic parameters of the resistance traits of Tegillarca granosa when infected with Vibrio harveyi, artificial infection experiments were performed on 22 T. granosa families using the immersion infection method with V. harveyi. Three genetic assessment models were used to evaluate the genetic parameters of the growth and resistance traits of T. granosa. At the end of the experiment, the mortality rate of T. granosa was close to 70%, and there was a significant difference in the survival rate between families (6.7%-60%). The results revealed that the range of heritability of resistance traits ranged from 0.17 ± 0.05 to 0.20 ± 0.07, which belonged to low and medium heritability. Pearson and Spearman correlations were used to conduct a correlation analysis of the breeding values of each line. The correlation coefficients estimated by the three models ranged from 0.953 to 0.998, thus exhibiting a significant positive correlation, which indicates that the three models exhibited a similar ability to estimate the genetic parameters of resistance traits. The phenotypic and genetic correlations between the growth and resistance traits ranged from -0.069 to -0.111 and from -0.412 to -0.545, respectively, revealing no significant negative correlation. The above results indicate that the resistance traits of T. granosa belonged to medium and low heritability traits, which are suitable for breeding by family selection. There was no significant difference in the genetic correlation between the two traits, indicating that the growth and resistance traits can be selected separately during breeding. This study supplements our knowledge of the correlation between the genetic parameters of T. granosa resistance to V. harveyi and provides a reference for breeding valuable strains of T. granosa with resistance to V. harveyi. |
| Key words: Tegillarca granosa Vibrio harveyi heritability genetic correlation resistance traits |
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