| 引用本文: | 姜珊,赵张琪,蒋之琛,李学刚,张均龙,张德超,沙忠利.黄河口潮间带沉积物夏秋季微生物群落特征及其对环境因素的响应[J].海洋科学,2025,49(5):13-23. |
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| 黄河口潮间带沉积物夏秋季微生物群落特征及其对环境因素的响应 |
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姜珊1,2, 赵张琪1,2, 蒋之琛1,2, 李学刚1,2, 张均龙1,2, 张德超1,2, 沙忠利1,2
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1.中国科学院海洋研究所 海洋生物分类与系统演化实验室, 山东 青岛 266071;2.中国科学院大学, 北京 100049
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| 摘要: |
| 为探究黄河口潮间带沉积物夏秋季微生物群落结构的组成及影响因素, 采集 8个站位的96个表层沉积物样品, 采用高通量测序技术, 分析夏秋季沉积物微生物群落结构的组成, 并探讨不同环境因子对微生物群落结构的影响。潮间带沉积物中最优势菌门为变形菌门(Proteobacteria), 较优势菌门为拟杆菌门(Bacteroidetes)和脱硫菌门(Desulfobacterota)。秋季和夏季的α多样性存在显著差异, 且秋季物种丰富度和均匀度均高于夏季。采用主成分分析(PCA)结合多元方差置换分析(PERMANOVA)研究β多样性, 发现夏秋季样本的微生物群落结构存在显著分离现象。通过LEfSe分析发现, 夏秋两季存在显著性的属水平类群共计37个, 其中夏季9个, 秋季28个。夏季和秋季的微生物丰度均与环境因子存在较强的相关性, 且不同季节的微生物受不同的环境因子影响, 表现出不同的丰度变化。经过FAPROTAX功能注释, 夏秋季丰度均较高的是和氮、硫代谢相关的功能。通过代谢差异进行分析发现, 秋季样本中显著上调的主要为自养代谢功能, 而夏季样本中显著上调的则为与分解代谢相关的功能。 |
| 关键词: 黄河口 潮间带沉积物 微生物群落结构 |
| DOI：10.11759/hykx20250110001 |
| 分类号:Q938 |
| 基金项目:黄河口海域海洋生物多样性调查评价项目(SDGP370000000202302001687) |
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| Microbial community structure of intertidal sediments in the Yellow River estuary and its environmental response from summer to autumn |
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JIANG Shan1,2, ZHAO Zhangqi1,2, JIANG Zhichen1,2, LI Xuegang1,2, ZHANG Junlong1,2, ZHANG Dechao1,2, SHA Zhongli1,2
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1.Department of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China
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| Abstract: |
| To investigate the composition of microbial communities in the intertidal sediments of the Yellow River Estuary during summer and autumn, 96 sediment samples were collected from 8 sampling sites. High-throughput sequencing technology was used to analyze microbial community composition from summer to autumn and to explore the influence of various environmental factors on the microbial community structure. Proteobacteria was the most dominant sediment bacterial phylum, followed by Bacteroidetes and Desulfobacterota. Significant differences in α-diversity were observed between autumn and summer, with both species richness and evenness being higher in autumn than in summer. Principal component analysis, combined with permutational multivariate analysis of variance, was used to assess β-diversity, and it revealed a significant separation in microbial community structures between summer and autumn samples. Using linear discriminant analysis effect size, 37 significantly different bacterial taxa at the genus level were identified between the summer and autumn groups, with 9 taxa enriched in summer and 28 in autumn. Microbial abundances in both summer and autumn were strongly correlated with environmental factors. Moreover, microbial communities in different seasons are influenced by different environmental factors and exhibit varying abundance changes. FAPROTAX functional annotation indicated that the higher abundance in summer and autumn was related to nitrogen and sulfur metabolism.genes related to nitrogen and sulfur metabolism were abundant in both summer and autumn. In addition, metabolic differences analysis showed that the significantly upregulated functions in autumn samples were mainly autotrophic metabolic functions, while the significantly upregulated functions in summer samples were related to catabolic metabolism. |
| Key words: the Yellow River Estuary intertidal sediments microbial community |
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