| 摘要: |
| 八放珊瑚作为深海海山生态系统中的关键类群,对维持深海生物多样性具有重要意义。然而,由于传统深海调查技术的限制,目前对深海海山八放珊瑚多样性的研究仍不充分。本研究利用环境DNA宏条形码技术结合影像分析对卡罗琳海山的八放珊瑚多样性进行综合分析,旨在分析环境DNA技术在海山调查中的可行性。通过环境DNA技术,共检测到65个分子种,涵盖2目、11科、19属;而影像技术则识别出29个形态种,涉及2目、6科、9属。两种方法均成功检测到热带西太平洋深海常见的八放珊瑚类群,如金柳珊瑚科、角柳珊瑚科、丑柳珊瑚科和棘柳珊瑚科,但在群落结构和物种组成上存在差异。环境DNA技术在检测隐匿物种和稀有类群方面表现出更高的灵敏度,而影像技术则在物种定量和形态特征分析上更具优势。本研究表明,环境DNA与影像技术的结合应用能够显著提升深海八放珊瑚多样性评估的全面性和可靠性,为深海生物多样性研究提供新的技术途径。 |
| 关键词: 环境DNA 八放珊瑚 卡罗琳海山 宏条形码技术 影像技术 |
| DOI：10.11759/hykx20250305002 |
| 分类号:X176 |
| 基金项目:国家自然科学基金(42176128、41930533、42306102);中国科学院战略性先导科技专项(XDB42000000) |
|
| Integrating environmental DNA and imaging techniques to study octocoral diversity on the Caroline Seamount |
|
ZHENG Xiaoyu1,2, XU Yu1, XU Kuidong1,2, LEI Yanli1,2, ZHAN Zifeng1,2
|
|
1.Laboratory of Marine Organism Taxonomy and Phylogeny, Shandong Province Key Laboratory of Marine Biodiversity and Bio-resource Sustainable Utilization, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China
|
| Abstract: |
| Octocorals are a group of macrobenthos, key to deep-sea seamount ecosystems and play a crucial role in maintaining deep-sea biodiversity. However, due to technological limitations in traditional deep-sea survey methods, studies on the octocoral diversity in deep-sea seamounts remain insufficient. This study combined environmental DNA (eDNA) metabarcoding with imaging techniques to comprehensively analyze octocoral diversity on the Caroline Seamount and evaluate the feasibility of eDNA technology in seamount surveys. eDNA analysis detected 65 molecular species and 19 genera, belonging to 11 families and 2 orders. In contrast, imaging techniques identified 29 morphospecies and 9 genera, belonging to 6 families and 2 orders. Both methods detected deep-sea octocoral groups, such as Chrysogorgiidae, Keratoisididae, Primnoidae, and Acanthogorgiidae, common to the tropical Western Pacific. They also revealed variations in the community structure and species composition. eDNA technology was more sensitive in detecting cryptic and rare species, whereas imaging methods exhibited greater advantages in quantitative analysis and morphological characterization. This study thus demonstrates that integrating eDNA and imaging techniques can significantly enhance the comprehensiveness and reliability of deep-sea octocoral diversity assessments, providing a new technical approach for deep-sea biodiversity research. |
| Key words: environmental DNA Octocorallia Caroline Seamount metabarcoding imaging techniques |
