引用本文:	申春琴,刘松,代娆,邢荣娥,陈晓琳,王铠露.不同脱乙酰度壳寡糖β-氨基丁酸衍生物的制备及其抗盐活性研究[J].海洋科学,2025,49(8):71-83.

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不同脱乙酰度壳寡糖β-氨基丁酸衍生物的制备及其抗盐活性研究
申春琴^1,2, 刘松^1,3, 代娆^1,2, 邢荣娥^1,3, 陈晓琳^1,3, 王铠露^1,2
1.中国科学院海洋研究所实验海洋生物学重点实验室, 山东青岛 266000;2.中国科学院大学, 北京 100049;3.青岛海洋科技中心海洋药物与生物制品功能实验室, 山东青岛 266237

摘要:

为探究脱乙酰度对壳寡糖β-氨基丁酸(COS-BABA)衍生物抗盐活性的影响,本研究通过可控乙酰化技术制备不同脱乙酰度壳寡糖,并通过羧基定向偶联策略合成4种不同脱乙酰度COS-BABA衍生物,其结构经红外光谱和核磁共振光谱进行表征。将衍生物作为外源诱导剂,对盐胁迫小麦进行浸种、浸根处理,系统探究壳寡糖脱乙酰度对衍生物抗盐活性的影响。结果表明,该系列衍生物可显著提升盐胁迫小麦的生长性能、光合色素含量、可溶性糖含量及抗氧化酶活性,并维持细胞膜稳定性,从而提高小麦幼苗抗盐能力。COS-BABA衍生物诱导抗盐活性受脱乙酰度显著影响,其中脱乙酰度为92%的衍生物(92-B)综合抗盐效果最佳,并且显著优于原料壳寡糖与β-氨基丁酸。本研究首次解析了脱乙酰度对壳寡糖β-氨基丁酸衍生物抗盐活性的调控作用,为壳寡糖分子精准改造及新型植物抗盐剂的开发提供了重要理论依据。

关键词: 壳寡糖 β-氨基丁酸脱乙酰度抗盐活性

DOI：10.11759/hykx20250410001

分类号:O636.1;TQ450.1

基金项目:山东省重点研发计划(重大科技创新工程)项目(2022CXGC020413)

Preparation of chitosan oligosaccharide β-aminobutyric acid derivatives with different degrees of deacetylation and their salt tolerance activity

SHEN Chunqin^1,2, LIU Song^1,3, DAI Rao^1,2, XING Ronge^1,3, CHEN Xiaolin^1,3, WANG Kailu^1,2

1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266000, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Functional Laboratory of Marine Drugs and Bioproducts, Qingdao Marine Science and Technology Center, Qingdao 266237, China

Abstract:

This study investigated the regulatory role of deacetylation degree (DD) on the salt tolerance-inducing potential of chitosan oligosaccharide (COS)- β-aminobutyric acid (BABA) derivatives. COSs with varying DDs were synthesized through controlled acetylation. Subsequently, four distinct COS-BABA derivatives were prepared via a carboxyl-directed coupling strategy. Structural characterization was performed using Fourier Transform Infrared Spectroscopy and Nuclear Magnetic Resonance spectroscopy. Moreover, the derivatives were used as exogenous inducers applied through seed soaking and root immersion to systematically evaluate the influence of DD on their ability to induce salinity tolerance in wheat grown under salt stress. The derivatives notably enhanced growth performance, photosynthetic pigment content, soluble sugar accumulation, and antioxidant enzyme activity in salt-stressed wheat seedlings while maintaining cell membrane integrity, thus synergistically improving salt tolerance. Notably, DD significantly influenced the salt tolerance-inducing activity, derivative with 92% DD demonstrating optimal efficacy, surpassing both raw COS and BABA treatments. To the best of our knowledge, this is the first systematic analysis to correlate DD with the salt tolerance-inducing ability of COS-BABA derivatives, providing a theoretical foundation for the precise modification of COS molecules and the development of novel plant-based “green” salt-resistance-producing agents.

Key words: chitosan oligosaccharide β-aminobutyric acid degree of deacetylation salt tolerance activity