| 引用本文: | 王旭雷,郑阵兵,郇丽,顾文辉,牛建峰,王立军,解修俊,张宝玉,王广策.我国南海紫杉状海门冬(Asparagopsis taxiformis)的物种鉴定及其溴仿合成关键酶基因克隆[J].海洋科学,2025,49(8):34-43. |
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| 我国南海紫杉状海门冬(Asparagopsis taxiformis)的物种鉴定及其溴仿合成关键酶基因克隆 |
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王旭雷1,2,3, 郑阵兵1,2,3, 郇丽1,2,3, 顾文辉1,2,3, 牛建峰1,2,3, 王立军1,2,3, 解修俊1,2,3, 张宝玉1,2,3, 王广策1,2,3
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1.中国科学院海洋研究所 实验海洋生物学重点实验室, 山东 青岛 266000;2.青岛海洋科技中心 海洋生物与生物技术功能实验室, 山东 青岛 266000;3.中国科学院海洋研究所 水产品种创制与高效养殖全国重点实验室, 山东 青岛 266000
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| 摘要: |
| 紫杉状海门冬(Asparagopsis taxiformis (Delile) Trevisan)可以作为反刍动物的饲料添加剂高效抑制甲烷排放,是一种具有巨大市场前景的潜在藻类养殖对象。然而,该物种在我国的相关研究还十分欠缺。为此,本文初步调查并采集了我国海门冬属海藻的配子体和孢子体样本,结合形态学和分子生物学证据鉴定为紫杉状海门冬,并详细描述了配子体和孢子体的形态学特征。基于cox2-3基因间隔序列的分子系统发育分析结果表明我国南海产紫杉状海门冬和夏威夷等地分布的种群亲缘关系密切,且同属于L4支系。同时,针对紫杉状海门冬溴仿合成关键酶基因,扩增并比较分析了我国样本与其他海域样本的溴仿合成关键酶基因的序列差异,表明编码溴仿合成关键酶的基因序列并不完全相同。相关研究结果阐释了紫杉状海门冬不同生活史阶段形态特征,初步证明了我国南海的紫杉状海门冬为L4支系,其溴仿合成关键酶编码基因也具有一定的独特性,该研究结果为深入开展我国紫杉状海门冬相关研究和资源开发利用提供了基础生物学数据支撑。 |
| 关键词: 红藻门 形态鉴定 系统发育分析 溴仿合成关键酶 反刍动物 |
| DOI：10.11759/hykx20250430004 |
| 分类号:Q949.25 |
| 基金项目:国家重点研发计划(2022YFF0802201);国家自然科学基金(42576098,42076089);国家现代农业产业技术体系(CARS-50);山东省“泰山学者”工程专项经费资助项目(tspd20210316) |
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| Species identification and cloning of the haloperoxidases coding genes of Asparagopsis taxiformis from the South China Sea |
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WANG Xulei1,2,3, ZHENG Zhenbing1,2,3, HUAN Li1,2,3, GU Wenhui1,2,3, NIU Jianfeng1,2,3, WANG Lijun1,2,3, XIE Xiujun1,2,3, ZHANG Baoyu1,2,3, WANG Guangce1,2,3
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1.Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266000, China;2.Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao 266000, China;3.State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266000, China
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| Abstract: |
| Asparagopsis taxiformis (Delile) Trevisan can effectively inhibit methane emissions in ruminants when used as a feed additive, and thus, cultivated seaweed potentially has substantial market prospects. However, research on this species remains limited in China. To address this, we collected gametophyte and sporophyte samples of Asparagopsis species from the South China Sea and performed a preliminary investigation. The morphological characteristics of the gametophytes and sporophytes were described in detail. Based on morphological evidence and molecular data, the gametophytes and sporophytes were identified as A. taxiformis. The results of molecular phylogenetic analysis based on the cox2-3 spacer revealed that the Chinese A. taxiformis population and that from Hawaii were closely related, both belonging to the L4 lineage. In addition, the key enzyme genes involved in bromoform synthesis in A. taxiformis were amplified, and differences in the sequence between samples from the South China Sea and those from other regions were compared and analyzed. The results indicated that the key enzyme-encoding gene sequences for bromoform synthesis were not entirely identical. These findings elucidated the morphological characteristics of the different life history stages of A. taxiformis, preliminarily confirming that the South China Sea population belongs to the L4 lineage, and demonstrated that the sequence of the key enzyme-encoding gene for bromoform synthesis was unique. This study provides fundamental biological data to support future research and resource development of A. taxiformis in China. |
| Key words: rhodophyta morphological identification phylogenetic analysis haloperoxidases ruminants |
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