引用本文:	郑丽华,韩笑天,徐秀丽.催化报告沉积-荧光原位杂交CARD-FISH技术在有害藻华研究中的应用前景及与主流分子检测技术的比较[J].海洋科学,2025,49(12):81-91.

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催化报告沉积-荧光原位杂交CARD-FISH技术在有害藻华研究中的应用前景及与主流分子检测技术的比较
郑丽华^1,2, 韩笑天², 徐秀丽¹
1.中国地质大学(北京)海洋学院, 极地地质与海洋矿产教育部重点实验室, 海南研究院, 北京 100083;2.中国科学院海洋研究所, 长江口海洋生态系统研究站, 山东青岛 266000

摘要:

环境变化和人类活动加剧了有害藻华(Harmful Algal Blooms, HABs)的频发,对生态系统和人类健康构成严重威胁。为了精准监测赤潮种的发生发展过程,发展高效、灵敏的检测技术至关重要。本文综述了催化报告沉积-荧光原位杂交技术(CAtalyzed Reporter Deposition Fluorescence In Situ Hybridization, CARD-FISH)在有害藻华量化研究中的应用;对比其他3种主流技术——高通量测序(High-Throughput Sequencing Technologies, HTS)、实时荧光定量PCR(Quantitative Polymerase Chain Reaction, qPCR)和荧光原位杂交(Fluorescence In Situ Hybridization, FISH), CARD-FISH凭借高灵敏和原位定量优势在有害藻华量化研究中显示出其重要价值,具有较好的应用前景及发展潜力。

关键词: 赤潮藻分子鉴定 CARD-FISH

DOI：10.11759/hykx20250725001

分类号:Q178.53

基金项目:山东省自然科学基金面上项目(ZR2022MD031)

Application prospects of catalyzed reporter deposition-fluorescence in situ hybridization in the research of harmful algal blooms and comparison with mainstream molecular detection techniques

ZHENG Lihua^1,2, HAN Xiaotian², XU Xiuli¹

1.School of Ocean Sciences, Key Laboratory of Polar Geology and Marine Mineral Resources, Ministry of Education, Hainan Institute, China University of Geosciences (Beijing), Beijing 100083, China;2.Yangtze Estuary Marine Ecosystem Research Station, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266000, China

Abstract:

Environmental changes and human activities have intensified the frequent occurrence of harmful algal blooms(HABs), posing serious threats to ecosystems and human health. Therefore, it is crucial to develop efficient and sensitive detection technologies for accurately monitoring the occurrence and development of HAB species and for precisely monitoring and managing microalgal communities. This article reviews the cutting-edge applications of catalyzed reporter deposition-fluorescence in situ hybridization(CARD-FISH) in the quantitative study of HABs and provides a comparative analysis with three other mainstream techniques: high-throughput sequencing technologies, quantitative real-time polymerase chain reaction, and fluorescence in situ hybridization. In addition, this paper highlights that CARD-FISH possesses unique advantages, such as high sensitivity and in situ quantification, making it highly valuable in the quantitative study of HABs. Furthermore, the article discusses the technique's application prospects and future development potential, providing theoretical support and technical reference for related research.

Key words: harmful algal blooms molecular identification CARD-FISH