| 引用本文: | 付敬强,张馨予,赵梅英,聂林蔚,郭团玉,骆轩,邹伟广,梁园,沈铭辉,游伟伟,柯才焕.方斑东风螺两个地理群体间杂交后代及其自交后代的遗传多样性和遗传结构分析[J].海洋科学,2026,50(1):26-34. |
| |
|
| |
|
|
| 本文已被：浏览 129次 下载 395次 |
码上扫一扫！ |
|
|
| 方斑东风螺两个地理群体间杂交后代及其自交后代的遗传多样性和遗传结构分析 |
|
付敬强1, 张馨予2,3, 赵梅英1, 聂林蔚2,3, 郭团玉1, 骆轩2,3, 邹伟广1, 梁园2,3, 沈铭辉4, 游伟伟2,3, 柯才焕2,3
|
|
1.厦门海洋职业技术学院海洋生物学院/海洋资源保护与生态治理福建省高等学校应用技术工程中心, 福建 厦门 361100;2.厦门大学 海水养殖生物育种全国重点实验室/海洋与地球学院, 福建 厦门 361102;3.福建省海洋经济生物遗传育种重点实验室, 福建 厦门 361102;4.海南省海洋与渔业科学院海南省热带海水养殖技术重点实验室, 海南 海口 571126
|
|
| 摘要: |
| 东风螺是我国重要的经济养殖贝类, 其杂交育种研究对于产业的可持续发展具有重要意义。为探究东风螺杂交子代的遗传多样性变化, 以方斑东风螺(Babylonia areolata)泰国群体(TT)和海南群体(HH)分别作为父母本, 采用2×2完全双列杂交设计, 获得TT(♀×♂)、TH(♀×♂)、HT(♀×♂)和HH(♀×♂)四种组合子代。利用14对微卫星标记对各组合子代进行遗传多样性和遗传变异分析。结果显示, 等位基因数(Na)、有效等位基因数(Ne)和Shannon’s信息指数(I)的变化范围分别为(3.40±0.39)~(4.80±0.63)、(2.73±0.24)~(3.42±0.40)、(1.06±0.09)~(1.25±0.12), 且这3项多样性指数在4种组合中排序均为HT>TH> HH>TT; 观测杂合度(Ho)与期望杂合度(He)的变化范围分别为(0.69±0.08)~(0.71±0.08)、(0.58±0.08)~ (0.65±0.05), 杂交子代略高于自交子代。此外, 4种组合间的遗传分化系数(Fst)变化范围为0.137 ~ 0.180, 其中HH与TT之间的遗传分化程度最大, HT与TH之间的分化程度最小。分子方差分析(AMOVA)结果显示, 4.0%的变异来源于各组合间, 17.0%的变异来源于组合内个体间, 79.0%来源于个体内。主坐标分析(PCoA)和Structure分析共同表明, HH与TT存在明显遗传差异, 杂交子代与两自交子代之间也呈现显著的的遗传结构差异。综上, 杂交提高了子代的遗传多样性, 杂交后代与自交群体表现出明显的遗传变异。本研究揭示了方斑东风螺杂交后代的遗传变化, 为其杂交育种提供了理论依据。 |
| 关键词: 方斑东风螺 杂交育种 微卫星标记 遗传多样性 |
| DOI：10.11759/hykx20251101001 |
| 分类号:S917.4 |
| 基金项目:海洋资源保护与生态治理福建省高等学校应用技术工程中心开放基金(2024-01); 国家自然科学基金(32202900); 厦门海洋职业技术学院高层次人才科研启动经费项目(KYG202545); 福建省科技计划项目(2025N0064) |
|
| Analysis of genetic diversity and structure between hybrid offspring and their inbred offspring of two geographical populations in Babylonia areolata |
|
Fu Jingqiang1, Zhang Xinyu2,3, Zhao Meiying1, Nie Linwei2,3, Guo Tuanyu1, Luo Xuan2,3, Zou Weiguang1, Liang Yuan2,3, Shen Minghui4, You Weiwei2,3, Ke Caihuan2,3
|
|
1.School of Marine Biology/Applied Technology Engineering Center of Fujian Provincial Higher Education for Marine Resource Protection and Ecological Governance, Xiamen Ocean Vocational College, Xiamen 361100, China;2.State Key Laboratory of Mariculture Breeding/College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China;3.Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, Xiamen 361102, China;4.Hainan Academy of Ocean and Fisheries Sciences, Hainan 571216, China
|
| Abstract: |
| Babylonia areolata is an economically important mariculture shellfish cultivated in China, with breeding research being of great significance for the sustainable development of the industry. To investigate the genetic changes among the offspring of B. areolata obtained via crossbreeding between the Thailand (TT) and Hainan (HH) populations of B. areolata as a 2×2 diallel cross to obtain four lineages: TT (♀×♂), TH (♀×♂), HT (♀×♂), and HH (♀×♂). Genetic diversity and variation in each combination of offspring were analyzed using 14 pairs of microsatellite markers. The number of alleles (Na), effective alleles (Ne), and Shannon’s information index (I) ranged from 3.40±0.39 to 4.80±0.63, 2.73±0.24 to 3.42±0.40, and 1.06±0.09 to 1.25±0.12, respectively. The order of significance of these indices for the four combinations was HT>TH>HH>TT. The observed (Ho) and expected (He) heterozygosity ranged from 0.69±0.08 to 0.71±0.08 and 0.58±0.08 to 0.65±0.05, respectively; the values were slightly higher for the hybrid than the self-propagated progeny. In addition, the genetic differentiation coefficients (Fst) of the four combinations ranged from 0.137 to 0.180. The degree of genetic differentiation between HH and TT was the maximum, and that between HT and TH was the least. Molecular analysis of variance (AMOVA) indicated that 4.0% variation was among populations, 17.0% variation was among individuals within populations, and 79.0% variation was within the individuals. PCoA and structural analysis revealed obvious differences between HH and TT, as well as differences in the genetic structure of the hybrids and parental populations. These results suggested that hybridization improved genetic diversity among offspring, which demonstrated obvious genetic variations compared with their parents. This study investigated the genetic profile of the offspring obtained from crossbreeding in B. areolata at the molecular level, which provided theoretical guidance for future breeding efforts. |
| Key words: Babylonia areolata hybrid breeding microsatellite marker genetic diversity |
|
|
|
|
|
|